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Exosome non-target metabolomics is a non-target metabolome detection technology based on orbitrap ultra-high resolution mass spectrometry, which can achieve accurate qualitative metabolites for the sample type of exosomes. Exosomes are a type of extracellular vesicles, and their contents include mRNA and miRNA, as well as proteins, lipids, and metabolites. In various diseases, exosomes provide new perspectives on changes in cell or tissue states, their detection in biological fluids may provide multi-component diagnostic information, and exosome-based material information transfer capabilities can also design related therapy.