Observation of cortical bone by dark-field transmission electron microscopy technology (CAT#: STEM-MIT-0015-LJX)

Introduction

Cortical bone is a dense mass of bone distributed over the peripheral surface of bone. Histologically, bone tissue can be divided into lamellar bone and non-lamellar bone. After one year of age, all bones in the human body are composed of lamellar bones. Mature bone tissue is often lamellar, called lamina.
Most of the mineral in bone is present in the form of "mineral structures", 5-6nm-thick, elongated plates which surround and are oriented parallel to collagen fibrils. Using dark-field transmission electron microscopy, mineral structures in ion-milled sections of cortical human bone cut parallel to the collagen fibrils can be viewed.

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Principle Applications Procedure Materials Notes Related Products

Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Cortical bone cells

Notes

Pay attention to air humidity
Voltage needs to be stabilized

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