Observation of fission yeast cells by transmission electron microscopy technology (CAT#: STEM-MIT-0014-LJX)

Introduction

Schizomyces is a simple single-celled organism with a gene group of 14 bM, about 4 times that of E. coli. A comparison of the genotype sequences showed that schizomyces was closer to mammalian cells than saccharomyces cerevisiae. The similarity between schizomyces and mammalian cells was greater than that between saccharomyces cerevisiae and mammalian cells in terms of pericycles, chromaticity structure and NRA splicing. Its conversion efficiency is also very high. Schizomyces has been successfully used in the study of double grouping, translation, RNA splicing, chromosome structure, mitosis, meiosis, cell cycle, and so on, and has become an increasingly attractive experimental system for the study of eukaryotic cell biology and molecular biology.

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Principle Applications Procedure Materials Notes Related Products

Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Fission yeast cells

Notes

Pay attention to air humidity
Voltage needs to be stabilized

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