Observation of platelet by transmission electron microscopy technology (CAT#: STEM-MIT-0017-LJX)

Introduction

Platelets are small pieces of cytoplasm liberated from the mature megakaryocyte cytoplasm of the bone marrow. Platelets play an important role in hemostasis. When blood is lost due to vascular trauma, it, together with clotting factors, reacts to the bleeding site of vascular injury through the action of clotting, thus forming clotting.
Platelet transmission electron microscopy (PTEM) is considered the gold standard test for assessing distinct ultrastructural abnormalities in inherited platelet disorders (IPDs).

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Principle Applications Procedure Materials Notes Related Products

Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Platelet

Notes

Pay attention to air humidity
Voltage needs to be stabilized

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