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Observing excretion of fluorescein by the liver under normal and abnormal conditions in vivo by the fluorescence microscope (CAT#: STEM-MIT-0177-LJX)

Introduction

The liver is an organ of vertebrate body which mainly metabolism function. Its main functions in the body are deoxidation, storage of liver sugars, synthesis of secretory proteins and so on.
Fluorescein is the substrate of luminous substances, which can make many living things fluorescent.




Principle

Fluorescence microscope is a basic tool of immunofluorescence cytochemistry. It is composed of light source, filter plate system and optical system. Is to use a certain wavelength of light to stimulate the specimen to emit fluorescence, through the objective lens and eyepiece system to enlarge the image to observe the specimen.

Applications

Studing the absorption, transportation, distribution and localization of chemical substances in cells
For observation and study of plant cells

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Malignant lung tumor cells

Notes

Operate in strict accordance with the manufacturer's instructions and do not change the procedure at will
The object of observation must be a specimen that fluoresces spontaneously or has been stained with fluorescent dye
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