POROS CaptureSelect™ AAVX PhyTip® Columns (500 µL Volume) Containing 20 µL Resin Per Column, PTT-95-20-33, Tecan (CAT#: STEM-C-4408-LGZ)

Adeno-associated virus (AAV) is a non-enveloped virus that can be engineered to deliver DNA to a target. The genome of the virus consists of a single-stranded linear DNA molecule of approximately 4.7 kb. Adeno-associated viruses are used as vectors to deliver various types of cargo to the cytoplasm and nucleus of various cells, including muscle, vascular endothelial cells, and neuronal cells.

For the downstream workflow of virus production, affinity chromatography purification was found to be effective in removing impurities such as plasmid DNA and host cell RNA/DNA. Empty capsids were then removed by anion exchange polishing.

CaptureSelectTM AAVX Resin has demonstrated binding reactivity to a range of AAV serotypes, as well as synthetic serotypes, including AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9 and AAVrh10.

CaptureSelectTM AAVX PhyTip® columns are compatible with our proprietary dual-flow chromatography and most 8-, 12- and 96-channel automated liquid handling robots.

CaptureSelect® is a trademark of Thermo Fisher Scientific Corporation.

Pack Size: 96

PhyNexus, now part of Biotage, developed PhyTip® columns in 2002 and has supported researchers in automated protein purification since then. PhyTip columns purify samples through repeated back and forth cycles, a process known as dual-flow chromatography that allows for complete binding interactions during each purification step.

High-capacity resin, high-recovery samples
Packed with high-capacity AAV affinity resins, our AAV PhyTip columns routinely generate purified samples of his-tagged proteins with sample recoveries of 60 - 80%.

Sample concentration and purification in one
Our unique packed resin bed column design results in the entire sample being bound to a small resin bed volume. Purified samples can be eluted in small volumes, resulting in highly concentrated samples.

Gentle Purification of Bioactive Proteins
PhyTip columns process samples through dual-flow chromatography, a gentle purification process that yields high levels of bioactive proteins.

15 minutes cleanse
Efficient purification via PhyTip columns allows for a complete purification protocol of 96 samples in as little as 15 minutes.

Validated Protocols
Our Protein A PhyTip columns use dual-flow chromatography, are true protein validated, and can be used in any automated liquid handling system.

POROS CaptureSelect™ AAVX PhyTip® Columns (200 µL Volume) Containing 5 µL Resin Per Column, PTT-92-05-33, Tecan (CAT#-STEM-C-4402-LGZ)
POROS CaptureSelect™ AAVX PhyTip® Columns (300 µL Volume) Containing 20 µL Resin Per Column, PTX-93-20-33, DD/Biotix (CAT#-STEM-C-4403-LGZ)
POROS CaptureSelect™ AAVX PhyTip® Columns (300 µL Volume) Containing 20 µL Resin Per Column, PTH-93-20-33, Hamilton (CAT#-STEM-C-4404-LGZ)
POROS CaptureSelect™ AAVX PhyTip® Columns (300 µL Volume) Containing 5 µL Resin Per Column, PTX-93-05-33, DD/Biotix (CAT#-STEM-C-4405-LGZ)
POROS CaptureSelect™ AAVX PhyTip® Columns (300 µL Volume) Containing 5 µL Resin Per Column, PTH-93-05-33, Hamilton (CAT#-STEM-C-4406-LGZ)
POROS CaptureSelect™ AAVX PhyTip® Columns (500 µL Volume) Containing 160 µL Resin Per Column, PTT-95-16-33, Tecan (CAT#-STEM-C-4407-LGZ)