POROS CaptureSelect™ IgM PhyTip® Columns (200 µL Volume) Containing 5 µL Affinity Media Per Column, PTT-92-05-30, Tecan (CAT#: STEM-C-4229-LGZ)

POROS CaptureSelect™ IgM Affinity Matrix purifies IgM antibodies by binding to their Fc region. The affinity medium consisted of CaptureSelect affinity ligands coupled to NHS-activated Sepharose 4 Fast Flow® resin (Cytiva). By binding to the unique domains of the Fc part of IgM, this affinity medium enables highly specific purification of human and mouse IgM without separation from complex sources such as plasma, serum, and cell culture supernatants IgG and IgA cross-react.

Affinity ligands for ThermoFisher's CaptureSelect™ media are based on recombinant antibody fragments of only 14 kDa in size and can be adapted for highly selective binding to certain moieties or antibodies and antibody fragments.

Pack Size: 96

PhyNexus, now part of Biotage, developed PhyTip® columns in 2002 and has supported researchers in automated protein purification since then. PhyTip columns purify samples through repeated back and forth cycles, a process known as dual-flow chromatography that allows for complete binding interactions during each purification step.

IgGs recovery 70 - 90%
Less protein loss during purification due to maximized sample binding. The result is very high sample recovery.

Sample concentration and purification in one
Our unique packed resin bed column design results in the entire sample being bound to a small resin bed volume. Purified samples can be eluted in small volumes, resulting in highly concentrated samples.

IgG purification for 15 minutes
Efficient purification via PhyTip columns allows for a complete purification protocol of 96 samples in as little as 15 minutes.

Compatible with all major liquid handling systems
Our PhyTip columns are compatible with all major liquid handling platforms, enabling you to use your existing systems, which you may already have in your laboratory for automated protein purification. If you do not have a system, we can provide an automated solution for protein purification with PhyTip columns.

POROS CaptureSelect™ IgM PhyTip® Columns (200 µL Volume) Containing 20 µL Affinity Media Per Column, PTV-92-20-30, Agilent Bravo (CAT#-STEM-C-4223-LGZ)
POROS CaptureSelect™ IgM PhyTip® Columns (200 µL Volume) Containing 20 µL Affinity Media Per Column, PTB-92-20-30, Beckman (CAT#-STEM-C-4224-LGZ)
POROS CaptureSelect™ IgM PhyTip® Columns (200 µL Volume) Containing 20 µL Affinity Media Per Column, PTR-92-20-30, MEA 2 (CAT#-STEM-C-4225-LGZ)
POROS CaptureSelect™ IgM PhyTip® Columns (200 µL Volume) Containing 20 µL Affinity Media Per Column, PTT-92-20-30, Tecan (CAT#-STEM-C-4226-LGZ)
POROS CaptureSelect™ IgM PhyTip® Columns (200 µL Volume) Containing 5 µL Affinity Media Per Column, PTB-92-05-30, Beckman (CAT#-STEM-C-4227-LGZ)
POROS CaptureSelect™ IgM PhyTip® Columns (200 µL Volume) Containing 5 µL Affinity Media Per Column, PTR-92-05-30, MEA 2 (CAT#-STEM-C-4228-LGZ)