Preparation of mouse brain tissue slices by freeze slicing technology (CAT#: STEM-CT-0002-LJX)

Introduction

Neural tissue consists of neurons and a variety of glial cells. There are hundreds of nuclear clusters in the brain, and the positions and functions of each cluster are very different. Therefore, morphological technique is of special significance to elucidate the structure and function of the nervous system. To study the tissue morphology and structure of the central nervous system, the histological sections should be prepared first. After the sections are made, the tissue structures can be distinguished under the microscope.Mouse brain tissue is soft and fragile, making it difficult to directly observe its morphology and structure.Fixing and slicing mouse brain tissue is beneficial for morphological analysis

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Principle Applications Procedure Materials Notes Related Products

Principle

Frozen section is the section made by freezing tissue quickly to a certain hardness under the condition of low temperature and constant cooling. Since tissues do not need any treatment, the chemical components of lipids, enzymes, glycogen, antigens, antibodies in tissues will not be affected and can be preserved, so it is suitable for the detection of lipids, enzymes, glycogen, antigens, antibodies and so on. It is commonly used for immunohistochemistry, immunofluorescence and in situ hybridization experiments.

Applications

Preparation of mouse nerve tissue section samples

Procedure

1.Mouse perfusion fixation
2.Dehydration of materials
3.Set the temperature of the cryotome
4.Freeze samples and embedding
5.Freeze slices

Materials

• Sample Type:
Mouse brain tissue

Notes

1.Check whether the blade is damaged
2.If there is fragmentation of brain slice during section, it indicates that the sample temperature is too low. If there is adhesion, the sample temperature is too high. The temperature of box and sample head should be adjusted accordingly to optimize the slicing effect.