Preparation of mouse brain tissue slices by paraffin slicing technology (CAT#: STEM-CT-0003-LJX)

Introduction

Neural tissue consists of neurons and a variety of glial cells. There are hundreds of nuclear clusters in the brain, and the positions and functions of each cluster are very different. Therefore, morphological technique is of special significance to elucidate the structure and function of the nervous system. To study the tissue morphology and structure of the central nervous system, the histological sections should be prepared first. After the sections are made, the tissue structures can be distinguished under the microscope.Mouse brain tissue is soft and fragile, making it difficult to directly observe its morphology and structure.Fixing and slicing mouse brain tissue is beneficial for morphological analysis

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Principle Applications Procedure Materials Notes Related Products

Principle

Paraffin slicing technology is one of the most widely used conventional sectioning techniques, which is used not only to observe the normal cell morphology, but also to study, observe and judge the morphological changes in pathology and forensics. Living cells or tissues are mostly colorless and transparent, and there is a lack of contrast between various structures in different tissues and cells, which is not easy to distinguish under a light mirror; Tissue will die soon after leaving the body and produce tissue corruption, losing the original normal structure. Therefore, the tissue should be fixed, paraffin embedded, sliced and stained to prevent cell tissue death, so as to be able to clearly identify its morphological structure.

Applications

Preparation of mouse nerve tissue section samples

Procedure

1.Mouse perfusion fixation
2.Draw materials
3.Gradient ethanol dehydration
4.Xylene transparent
5.Wax immersion
6.Slice
7.Glue the wax sheet to the slide and dry it

Materials

• Sample Type:
Mouse brain tissue

Notes

1.The slide shall be cleaned with acid and treated with anti-peeling. Commercial anti-slip slides can be purchased or made from polylysine, APES or gelatin.
2.Pay attention to the direction of sections in the patch, and it is better to number them in sequence for the convenience of HE staining, immunohistochemical staining or in situ hybridization staining.