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Neural tissue consists of neurons and a variety of glial cells. There are hundreds of nuclear clusters in the brain, and the positions and functions of each cluster are very different. Therefore, morphological technique is of special significance to elucidate the structure and function of the nervous system. To study the tissue morphology and structure of the central nervous system, the histological sections should be prepared first. After the sections are made, the tissue structures can be distinguished under the microscope.Mouse brain tissue is soft and fragile, making it difficult to directly observe its morphology and structure.Fixing and slicing mouse brain tissue is beneficial for morphological analysis.