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Preparation of mouse brain tissue slices by vibration slicing technology (CAT#: STEM-CT-0001-LJX)

Introduction

Neural tissue consists of neurons and a variety of glial cells. There are hundreds of nuclear clusters in the brain, and the positions and functions of each cluster are very different. Therefore, morphological technique is of special significance to elucidate the structure and function of the nervous system. To study the tissue morphology and structure of the central nervous system, the histological sections should be prepared first. After the sections are made, the tissue structures can be distinguished under the microscope.Mouse brain tissue is soft and fragile, making it difficult to directly observe its morphology and structure.Fixing and slicing mouse brain tissue is beneficial for morphological analysis.




Principle

Vibrating section is to use a vibrating microtome to cut fresh tissue (not frozen) into 20-400μm thick slices, with floating method in the reaction plate for immunohistochemical staining (referred to as immunohistochemical). The main advantages of this method are that the tissue is not frozen, there is no ice crystal formation and no tissue antigen destruction. Before immunohistochemical staining, the damage to the antigen caused by tissue dehydration, transparency and embedding can be avoided, and the lipid soluble substances and cell membrane antigens can be preserved well in the tissue.

Applications

Preparation of mouse nerve tissue section samples

Procedure

1.Tissue embedding
2.Install the slicer and blade
3.Fix tissue block
4.Slice the tissue block

Materials

• Sample Type:
Mouse brain tissue

Notes

1.When slicing, ensure a dark room environment and avoid bright light.
2.When repairing the tissue block, it is necessary to clean the AGAR in front of the tissue block to prevent damage to the blade and affect the quality of the brain piece.
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