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The prokaryotic protein expression system generally uses Escherichia coli as the host cell. Due to the advantages of fast cell proliferation, high expression, convenient purification, good stability, strong anti-pollution ability and low cost, the prokaryotic protein expression system is the most mature and reliable, and it is also the most commonly used and most economical expression system in protein expression experiments. The Escherichia coli expression system is the most commonly used protein expression system. The advantages include clear genetic background, low cost, high expression level, easy amplification and short time, etc. The disadvantages are that it is easy to form inclusion bodies and cannot perform post-translational modification. This system is suitable for expressing prokaryotic proteins or eukaryotic proteins that do not require post-translational modification, especially small molecular proteins.