Protein-Ligand Interaction NMR Service Based on 700 MHz Spectrometer

STEMart provides specialized Protein-Ligand Interaction NMR services using high-performance 700 MHz spectrometers, including the Bruker Avance III 700 MHz and Bruker Avance NEO 700 MHz systems with Cryoprobe technology. These advanced instruments allow us to study molecular interactions at an atomic level, essential for drug discovery, structural biology, and biochemical research. With high sensitivity and resolution, our 700 MHz spectrometers offer detailed insights into protein-ligand binding dynamics and affinities.

Our 700 MHz NMR technology operates on the principles of nuclear magnetic resonance, enabling us to observe interactions between proteins and small molecules in solution. Techniques such as saturation transfer difference (STD), transferred NOE (trNOE), and chemical shift perturbation (CSP) provide comprehensive data on binding sites, interaction strength, and molecular orientation. Widely applied in fields like pharmacology, molecular biology, and biophysics, NMR-based protein-ligand analysis provides a non-invasive, solution-based method for understanding molecular recognition.

Leveraging our state-of-the-art 700 MHz NMR spectrometers and skilled team, STEMart offers a complete suite of protein-ligand interaction services. These include binding affinity measurements, epitope mapping, and kinetic analysis, delivering precise and reliable data to support researchers in drug development, protein engineering, and functional studies.

What We Offer

• Binding Affinity Analysis

Measures the strength of protein-ligand binding interactions, providing essential data for drug design and molecular targeting.

• Epitope Mapping

Identifies specific binding regions on the ligand or protein, aiding in the understanding of binding mechanisms and molecular recognition.

• Chemical Shift Perturbation Studies

Monitors shifts in resonance frequencies upon binding, offering insights into binding sites and structural changes in the protein-ligand complex.

• Kinetic Analysis of Binding Interactions

Provides dynamic information on binding rates, supporting research in pharmacokinetics and biomolecular interactions.

• Structure-Activity Relationship (SAR) Studies

Assesses the effects of ligand modifications on binding, supporting optimization in drug discovery.

Why Choose Us

• High Sensitivity for Weak Interactions

Our 700 MHz spectrometers provide high sensitivity, allowing for the detection of low-affinity interactions crucial for early-stage drug discovery.

• Expert Interpretation and Analysis

Our team of experienced NMR scientists ensures accurate interpretation, delivering reliable data to advance research in structural biology.

• Comprehensive Data Reporting

We offer flexible reporting options, tailored to meet specific research needs, supporting clear and detailed data analysis.

• Non-Invasive, Solution-Based Methodology

Our solution-state NMR approach preserves natural protein-ligand interactions, providing accurate insights into biological systems.

Our Resources

Bruker Avance III 700 MHz NMR

This high-resolution 700 MHz spectrometer from Bruker features advanced Cryoprobe technology, enhancing sensitivity for protein-ligand studies. Its robust capabilities support detailed interaction analysis, making it ideal for binding affinity and structural studies in drug discovery.

Bruker Avance NEO 700 MHz with Cryoprobe

The Bruker Avance NEO 700 MHz system, equipped with Cryoprobe, provides exceptional sensitivity and resolution for complex biomolecular interactions. Its advanced features enable accurate and reliable protein-ligand binding analysis, supporting research in molecular biology and pharmacology.

STEMart provides high-quality NMR services tailored to meet the specific needs of our clients. Our experienced specialists use advanced equipment and follow strict quality control measures to ensure accurate and reliable results. Contact us to learn more about our NMR services and how we can help you achieve your research goals.