Protein Thermal Shift Assay (PTSA) to measure protein thermal stability (CAT#: STEM-MB-0238-WXH)

Introduction

PTSA is a thermo-denaturation assay to measure protein thermal stability. Protein thermal denaturation temperature changes based on different conditions the protein is under. PTSA is widely used in drug discovery, protein engineering, structural genomics studies and screening of optimized conditions for protein purification, storage, crystallization, characterization, etc.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Measure protein thermal stability.
• Widely used in drug discovery, protein engineering, structural genomics studies and screening of optimized conditions for protein purification, storage, crystallization, characterization, etc.

Procedure

Various approaches used for PTSA include reporter dyes (thermofluor, thiol-specific dyes CPM, rigidity sensitive dyes DCVJ), intrinsic tryptophan fluorescence lifetime, fluorescence wavelengths, static light scattering, Fast Parallel Proteolysis (FastPP), Cellular Thermal Shift Assay (CETSA), Fluorescence-detection Size-Exclusion Chromatography-based ThermoStability assay (FSEC-TS) and Radio ligand Binding Thermo stability Assay (RBTA).

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