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PROTEINDEX™ Glutathione Agarose 4 FF, Prepacked Cartridge, 1 x 5 mL, 11-0200-1x5ML, Marvelgent biosciences (CAT#: STEM-C-3725-LGZ)

Cat Number: STEM-C-3725-LGZ

Application: For easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect and mammalian cells.

Model: 11-0200-1x5ML

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Description

Agarose 4FF is an affinity chromatography medium for simple one-step purification of recombinant glutathione s-transferase (GST) fusion proteins and other glutathione conjugates expressed in E. coli, insect and mammalian cells protein. Rigid, highly cross-linked support suitable for purification scale applications. Prepackaged Glutathione Sepharose 4FF cartridges are ready-to-use and compatible with syringes, AKTA, and other peristaltic pump systems.

Specification

Size: 1 x 5 mL
Ligand: Glutathione coupled with a 12-atom linker
Ligand concentration: 120 - 320 μmol Glutathione/mL medium
Binding capacity: >10 mg GST (26 kDa)/mL medium
Matrix: 4% highly cross-linked agarose
Particle size: 45 μm - 165 μm
Maximum pressure: 0.3 MPa (3 bar, 43 psi)
Recommended flow rate: 300 - 500 cm/hour
pH stability: 3-12
Storage temperature: 2 - 8°C
Storage buffer: 20% ethanol as preservative

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