PROTEINDEX™ rProtein A Agarose 4FF, Prepacked Cartridge, 5 x 1mL, 11-0204-5x1ML, Marvelgent biosciences (CAT#: STEM-C-3689-LGZ)

PROTEINDEX™ rProtein A Sepharose 4 Fast Flow is an affinity chromatography medium designed for simple one-step purification of immunoglobulins, especially of the IgG isotype, and fusion proteins containing the Fc region.

Protein A is a bacterial cell wall protein isolated from Staphylococcus aureus that binds to the Fc region of most immunoglobulins through interaction with the heavy chain. Native protein A contains five high-affinity IgG-binding domains and many domains with unknown functions. Recombinant protein A coupled to agarose resin was produced in Escherichia coli and contained five high-affinity IgG binding domains, with other non-essential domains removed to reduce non-specific binding. rProtein A is basically the same as natural protein A, and can bind with high affinity to the Fc region of human, rabbit and other animal IgG.

Through the proprietary ProteIndex™ technology, the immobilization of recombinant protein A on 4% highly cross-linked agarose resin is very stable, resulting in high yields in laboratory preparations and scale-up applications.

Size: 5 x 1mL
Ligand: Recombinant protein A produced in E.coli
Binding capacity: >40 mg human IgG/mL medium
Matrix: Highly cross-linked 4% agarose
Particle size: 45 μm - 165 μm
Maximum pressure: 0.3 MPa (3 bar)
Chemical stability: All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.
Recommended flow rate: Gravity flow and medium pressure, 30 - 300 cm/hour
pH stability: Long term: pH 3 - 10; Short term: pH 2 - 11.
Storage temperature: 2 - 8°C
Storage buffer: 20% ethanol

Large volume, > 40 mg human IgG / mL of settled resins
High selectivity, high purity
Ligand leakage is minimal and reusable
Ready to use, compatible with standard automation systems

Chromatography Columns, 1 Adjustable End, 5-7bar, 8mm ID, 300mm Length, 7bar, GB-8-300-1TE, G-BIOSCIENCES (CAT#-STEM-C-3374-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 8mm ID, 400mm Length, 7bar, GB-8-400-1TE, G-BIOSCIENCES (CAT#-STEM-C-3375-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 8mm ID, 500mm Length, 7bar, GB-8-500-1TE, G-BIOSCIENCES (CAT#-STEM-C-3376-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 8mm ID, 600mm Length, 7bar, GB-8-600-1TE, G-BIOSCIENCES (CAT#-STEM-C-3377-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 8mm ID, 700mm Length, 7bar, GB-8-700-1TE, G-BIOSCIENCES (CAT#-STEM-C-3378-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 8mm ID, 800mm Length, 7bar, GB-8-800-1TE, G-BIOSCIENCES (CAT#-STEM-C-3379-LGZ)