Proteins Production

Cell-free protein synthesis (CFPS) refers to production of proteins outside of living cells. It involves the use of cellular extracts that contain all the necessary components for protein synthesis, including ribosomes, amino acids, tRNA, and enzymes. The whole process of CFPS involves two main steps: transcription (TK) and translation (TL). This technology offers several advantages over traditional cell-based protein synthesis:

• Open system without cell walls allows continuous monitoring and specific adaptation of reaction conditions;
• Introduction of non-natural amino acids enables production of proteins with completely new biochemical properties;
• Able to synthesize proteins that are toxic to cells.

CFPS is a versatile and powerful technology that has the potential to revolutionize the way proteins are produced and engineered for various applications. However, low protein yields and high reagents costs remain limitations of CFPS.

Microfluidic technology is being increasingly used in CFPS due to its ability to monitor and regulate the relevant reaction parameters with integrated sensors. The application of microfluidic technology in CFPS offers significant advantages:

• TK and TL can be performed at the same time in separate reaction compartments with continuously optimal conditions for time saving;
• Quasi-continuous transfer of RNA to the TL compartment overcome the problems due to RNA degradation and hydrolysis;
• Implementation of multiple steps including TK, immobilization, transfer and elution of mRNA, and TL into one microfluidic device significantly reduces contamination risk, reagent consumption, manpower, costs, and process time.

What We Can Do for You

STEMart provides a microfluidic platform for automated cell-free protein synthesis.

• Rapid screening of various reaction conditions and parameters for to quickly optimize protein production
• In vitro efficient production of membrane proteins, cytotoxic proteins and “difficult-to-express” proteins
• Integrated protein purification and analysis to streamline the protein synthesis process and facilitate downstream analysis

For more information about our proteins production service, please contact us.