ProteoSure™ Recombinant Human Trypsin, 10 mg, 14-RHT-10MG, Marvelgent biosciences (CAT#: STEM-GT-3030-LGZ)

Trypsin is a member of the serine protease family. Trypsin cleaves the polypeptide at the C-terminus of the lysine and arginine amino acid residues. The optimal pH value of trypsin is between 7.8 and 8.7. The enzyme is inhibited by serine protease inhibitors such as PMSF and metal chelators such as EDTA.

Recombinant human trypsin is a genetically engineered protein expressed in Escherichia coli and purified by high pressure liquid chromatography. The amino acid sequence is identical to that of human trypsin 2. No contaminating enzyme activity from carboxypeptidase A or chymotrypsin. No protease inhibitors such as PMSF are used in the production process.

Size: 10 mg
CAS #: 9002-07-07
Enzyme Commission #: 3.4.21.4
Molecular weight: 23.8 kDa
Origin of species: Human
Source: E.coli
Purified by: HPLC
Physical form: White or white-like lyophilized powder
Specific activity: ≥ 2500 USP unit/mg protein
Unit definition: One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0 mL at pH7.6 and 25℃, with BAEE as a substrate (1 cm light path).
Purity: ≥ 95% by HPLC
Contaminant activity: No chymotrypsin, carboxypeptidase A, or other protease contaminants.
Storage: Store in a sealed container at 2 - 8°C. The product is stable for 24 months.

Once reconstituted, it should be stored at -20°C. It is stable for 24 months and greater than 90% enzymatic activity remains after 10 repeated freezing-and-thawing.

Intended use: For research or processing only. Not intended for any human or animal diagnostic or therapeutic use.

Animal source free
The use of recombinant human trypsin eliminates the potential risk of contamination with viruses and any other potential variable factors found in trypsin of animal origin. Recombinant Human Trypsin is 100% AOF and produced in an ISO9001 certified facility.

Human recombination
This product is recombinant human trypsin. The amino acid sequence is identical to that of human trypsin 2. Suitable for applications requiring low immunogenicity components.

Stable
Recombinant human trypsin is supplied as a sterile lyophilized powder. It minimizes the risk of contamination and loss of activity during transport and storage.

Highly pure
Recombinant human trypsin has superior specificity and purity.
No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
HPLC method purity greater than 95%.

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