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Pyroptosis Pathway Assay (CAT#: STEM-MB-0343-WXH)

Introduction

Pyroptosis refers to the autonomous and orderly death of cells controlled by genes in order to maintain the stability of the internal environment. Pyroptosis is different from cell necrosis. Pyroptosis is not a passive process, but an active process. It involves the activation, expression and regulation of a series of genes. It is not a kind of autologous damage under pathological conditions. Phenomenon, but a process of death actively striving for better adaptation to the living environment.
Pyroptosis is a basic life phenomenon widely existing in the biological world, and it plays a very important role just like cell growth, development, and proliferation. At present, it is believed that the extracellular stimulus that induces pyroptosis must be transmitted through a series of intracellular signals, and the selective breakage of DNA between nucleosomes is one of its important signs.




Principle

The intracellular information transmission pathway of pyroptosis can be roughly divided into two stages, namely the induction stage and the implementation stage. There are endogenous and exogenous factors that induce pyroptosis in the induction stage of pyroptosis. Endogenous factors include the activation of pyroptosis-inducing mechanisms (such as Fas ligand, tumor necrosis factor, etc.) and the inactivation of inhibitory mechanisms (proliferative factors such as growth factors, hormones, receptor factors, etc.).

Applications

To study the effects of drugs or therapies on pyroptosis signaling pathways
To study the effect of each virus on pyroptosis signaling pathway
To study the regulation mechanism of pyroptosis signaling pathway in disease

Procedure

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Notes

Detectable targets: Ask1, PI3K, ERK1, ERK2, PKC, NIK, JNK, p53, Cdc2, Ak1, Bad, NFκB, Bcl-2, Bcl-xL, Htr, A2, clAP, Apaf1, GSK-3, FKHR, AIF, APP, Rock, Caspase-9, TRAF3, Tak1, IRF9, MEKK1, p38, IPS-1, TRAF5

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