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Quantification of Insulin and Calf Thymus DNA by a Resonance Rayleigh Scattering Method (CAT#: STEM-ST-0040-YJL)

Introduction

Bovine insulin consists of 51 amino acids in two peptide chains: the A chain with 21 amino acids (Gly A1-Asn A21) and the B chain with 30 amino acids (Phe B1-Ala B30). The A and B chains are connected by two disulfide bridges. Insulin is a physiologically important protein hormone for the control of glucose metabolism and the treatment of diabetes. Therefore, understanding the interaction between insulin and small molecules or biomacromolecules and the development of new determination methods for insulin are of significance for biochemical, pharmacological, clinical and analytical applications.
Calf thymus DNA (DNA from calf thymus) is high-quality double-stranded template DNA isolated from the thymus of male and female calves. Calf thymus DNA can be used to study the interaction between DNA and active molecules.




Principle

Resonance Rayleigh scattering (RRS) is similar to Rayleigh scattering in nature. Resonance Rayleigh scattering is a special elastic scattering produced when the wavelength of Rayleigh scattering (RS) is located at or close to its molecular absorption band. The key to generating RRS is: when the scattering is at or close to the absorption band of the scattering molecule, since the electron absorbs the electromagnetic wave at the same frequency as the scattering frequency, the electron strongly absorbs the photon energy due to resonance and re-scatters. Its scattering intensity is several orders of magnitude higher than that of pure Rayleigh scattering, and it no longer obeys the Rayleigh law of I∝λ-4. This absorption-rescattering process is called resonance Rayleigh scattering (RRS).

Applications

Resonance Rayleigh scattering is used to the study of aggregation of chromophores on biological macromolecules and the determination of biological macromolecules such as nucleic acid, proteins and heparin, further, it has been used in the determination of trace amounts of inorganic ions and the cationic surfactant by means of ion association reactions with some dyes. In addition, it has been applied to the study of nanoparticles in liquid and the determination of β-cyclodextrin inclusion constant and the critical micelle concentration of surfactant.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Rayleigh scattering measurement system
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