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Caffeine (CAF) or 1,3,7-trimethylxanthine, is one of the most studied and consumed stimulants in the world. CAF is present in a wide variety of foods and beverages, as well as in nearly 60 plant species.
This service provides a capillary electrophoresis method for the analysis of paracetamol (PAR), caffeine (CAF) and codeine (COD) phosphate from pharmaceutical dosage forms. Good results were obtained by using a background electrolyte solution consist of 25 mM (pH 8.5) phosphate buffer solution containing 10% methanol. The separation was performed through an uncoated fused-silica capillaries (50 μm internal diameter, 56 cm total length, 47.5 cm effective length) at 25ºC with the application of 10 seconds of hydrodynamic injection at 40 mbar pressure and a potential of 27 kV. Detection wavelength was chosen 210 nm.