Rapid determination of atrazine residues in foods by supercritical fluid chromatography (CAT#: STEM-CT-0068-LJX)

Introduction

It is an accepted fact that many food products that we eat today have the possibility of being contaminated by various chemicals used from planting to processing. These chemicals have been shown to cause illnesses for which some concerned government agencies have instituted regulatory mechanisms to minimize the risks and the effects on humans. It is for these concerns that reliable and accurate rapid determination techniques are needed to effect proper regulatory standards for the protection of people's nutritional health. This service, therefore, determinates of atrazine (commonly used in agricultural as a herbicide) residues in foods using supercritical fluid chromatography (SFC) and enzyme-linked immunosorbent assay (ELISA) techniques.

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Principle Applications Procedure Materials Notes Related Products

Principle

Supercritical fluid is a substance that has both gaseous and liquid properties above the critical point. Supercritical fluids have the advantages of high diffusion coefficient, low viscosity, adjustable solubility and high vapor phase density, so they can provide efficient mass spectrometry ionization and separation results.
Supercritical fluid chromatography (SFC) is an efficient separation technique that uses supercritical fluid as a mobile phase. The samples are packed into short tubes or SPE columns, and the samples are compressed and regulated by supercritical fluid to obtain good solubility. The sample components are then separated by column interaction, thus achieving the separation of different compounds.

Applications

For efficient separation of substances
Widely used in biology, chemistry, environmental protection and other fields

Procedure

1. Sample injection
2. The high pressure pump increases the pressure of the sample and mobile phase
3. The sample and mobile phase enter the chromatographic column
4. Flow limiter assists sample separation

Materials

• Sample Type:
Foods

Notes

1. In the process of separation, the control of pressure and temperature of supercritical fluid is very important, which affects the properties and separation efficiency of supercritical fluid.
2. In addition, the selection of the appropriate column, packing and moving equivalent factors will also affect the separation effect.

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