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Rapid Screening of Multiple β-Globin Gene Mutations by RT-qPCR (CAT#: STEM-MT-0009-LGZ)

Introduction

Thalassemia is one of the most common hereditary diseases in the world. Blood routine and hemoglobin analysis are the main screening methods for thalassemia. Clinically, it is mainly divided into α-thalassemia and β-thalassemia, which is a hereditary hemolytic anemia caused by the deficiency or absence of synthesis of α or β globin chain due to the mutation or deletion of α or β globin gene, and the life span of red blood cells is shortened.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Application to Carrier Screening and Prenatal Diagnosis of Thalassemia Syndromes

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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