Recombinant Human B7-1 Protein, Seafrom (CAT#: STEM-CT-0130-ZJF)

The Recombinant Human B7-1 Protein manufactured by Seafrom is suitable for T-cell activation.
B7-1 and B7-2, along with their receptors CD28 and CTLA-4, constitute a crucial co-stimulatory signaling pathway that regulates T and B cells. While CTLA-4 and CD28 can bind to the same ligands, CTLA-4 has an affinity for B7-1 and B7-2 that is 20-100 times higher than CD28, leading to the downregulation of immune responses. B lymphocyte activation antigen B7-1 (referred to as B7), also known as differentiation antigen 80 (CD80), is a member of the immunoglobulin superfamily expressed on the surface of activated B cells, activated T cells, macrophages, and dendritic cells. It serves as a ligand for two different proteins on the surface of T cells: CD28 (for self-regulation and intercellular binding) and CTLA-4 (for attenuated regulation and cell detachment). CD80 acts in conjunction with CD86 on initial T cells, and CD80 plays a role in inducing innate immune responses by activating the NF-κB signaling pathway. Therefore, CD80 is considered an effective target for the treatment of autoimmune diseases and various cancers.
Storage Information:
The lyophilized (freeze-dried) form can be stored at 4°C for 24 months, while the reconstituted liquid can be stored at -20°C for 6-12 months, with a recommendation to avoid repeated freeze-thaw cycles.

Expression Host: Human HEK293 cells
Source: Human
Synonyms: CD80, LAB7, B7, T-lymphocyte activation antigen CD80, CD_antigen=CD80, CTLA-4 counter-receptorB7.1, CD28LG1, CD28LG, Activation B7-1 antigen, BB1
Protein Sequence: The DNA sequence encodes human B7-1 (NP_005182.1), expressed with a His tag at the C-terminus.
Molecular Weight: Recombinant human B7-1 protein contains 209 amino acids, with a predicted theoretical molecular weight of 25.2 kD.
Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis.
Endotoxin: <0.1 EU/μg
Biological Activity: The binding activity was determined using ELISA. Immobilized human CTLA-4 (FC tag) at 10 μg/mL (50 μL/well) can bind to human B7-1 (His tag) in a linear range of 0.05-1.0 μg/mL.
Purification Method: Chromatographic purification
Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.

Optimal Stability
Gmp-Grade Quality
Low Batch-to-Batch Variability
Exceptional Specificity
Robust Bioactivity
Efficient and Reliable Performance

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