Recombinant Human CD27 Ligand/CD70 (hFc Tag), Yisheng (CAT#: STEM-CT-0199-ZJF)

The Recombinant Human CD27 Ligand/CD70 (hFc Tag) manufactured by Yisheng is suitable for studying CD27 Ligand/CD70-mediated immune responses and its potential in cell therapy.
CD27 ligand (CD27L), also known as CD70, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF7). Human CD27L encodes a 193 aa residue protein with a 17 aa N-terminal cytoplasmic domain, a 21 aa transmembrane domain, and a 155 aa C-terminal extracellular domain. CD27L is expected to exist as non-covalent homotrimers. Constitutively expressed on medullary thymic epithelial cells (mTECs) in human and mouse, and on certain unconventional APCs in the mouse intestine, CD70 is exclusively expressed on DCs, B cells, conventional- and regulatory T cells, and NK cells but only after their activation. Its expression is highly regulated by antigen through Pattern Recognition Receptors (PRRs), T cell and B cell antigen receptors, and further tuned by cytokines such as IL-1 alpha.

Synonyms: CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG;Ki-24 antigen; TNFSF7G
Uniprot No.: P32970-1
Source: Recombinant Human CD27 Ligand/CD70 Protein is expressed from HEK293 Cells with hFc at the N-terminal. It contains Gln39-Pro193.
Molecular Weight: The protein has a predicted MW of 42.7 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: > 95% as determined by SDS-PAGE and HPLC.
Activity: ELISA Data: Immobilized Human CD27, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 37.9ng/ml determined by ELISA.
Endotoxin: < 1.0 EU per 1μg of the protein by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Shipped with ice pack.
Stored at -20℃ to -80℃ for 1 year.
2-7 days, 2 to 8 ℃ under sterile conditions after reconstitution.
3 months, -20 ℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
For safety and health, please wear lab coats and disposable gloves for operation.

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