Recombinant Human CD30 Ligand /TNFSF8 (mFc Tag), Yisheng (CAT#: STEM-CT-0208-ZJF)

The Recombinant Human CD30 Ligand /TNFSF8 (mFc Tag) manufactured by Yisheng is suitable for studying CD30 Ligand/TNFSF8-mediated immune responses and potential applications in cell therapy.
CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes, and is also constitutively expressed on granulocytes and medullary thymic epithelial cells. The specific receptor for CD30L is CD30/TNFRSF8, a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and cell death by apoptosis. It can act as a co-stimulatory molecule in thymic negative selection and may play a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. Human and mouse CD30 ligand cDNAs share 70% sequence homology.

Synonyms: CD153; CD30 Ligand; CD30L; CD30-L; CD30LGMGC138144; TNFSF8; CD30LG
Uniprot No.: P32971-1
Source: Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from HEK293 Cells with mFc tag at the N-terminal. It contains Gln63-Asp234.
Molecular Weight: The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: > 95% as determined by SDS-PAGE and HPLC
Activity: ELISA Data: Immobilized Human CD30, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 0.12μg/ml determined by ELISA.
Endotoxin: < 1.0 EU per 1μg of the protein by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Shipped with ice pack.
Stored at -20℃ to -80℃ for 1 year.
2-7 days, 2 to 8 ℃ under sterile conditions after reconstitution.
3 months, -20 ℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
For safety and health, please wear lab coats and disposable gloves for operation.

Recombinant Human CEACAM-6/CD66c (His-Avi Tag), Yisheng
Recombinant Human Her3/ErbB3 (hFc Tag), Yisheng
Recombinant Human SLAMF7/CRACC/CD319 (hFc Tag), Yisheng
Recombinant Human VEGFR2/KDR (mFc Tag), Yisheng