Recombinant Human CD40 Ligand Protein, Seafrom (CAT#: STEM-CT-0139-ZJF)

The Recombinant Human CD40 Ligand Protein manufactured by Seafrom is suitable for immune cell activation and regulation.
CD40 ligand (CD40L), also known as CD154, TNFSF5, and T-cell antigen Gp39, is a single-pass type I membrane protein belonging to the TNF superfamily of molecules. CD40 ligand is primarily expressed on activated CD4+ T lymphocytes but is also present in other cell types, including platelets, mast cells, macrophages, eosinophils, NK cells, B lymphocytes, and non-hematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). While all monomeric, dimeric, and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand, through the oligomerization of cell surface CD40, exhibits the most effective biological activity, a common characteristic of the TNF receptor family.
CD40 ligand binds to CD40 on antigen-presenting cells (APCs), leading to various effects depending on the target cell type. Generally, CD40 ligand acts as a co-stimulatory molecule and induces activation in APCs, related to the stimulation of T-cell receptors by MHC molecules on APCs. CD40 ligand has three binding partners: CD40, α5β1 integrin, and αIIBβ3. It regulates the function of B cells by binding to CD40 on the surface of B cells. Defects in this gene result in the failure of immunoglobulin class switching and are associated with hyper IgM syndrome.
Storage information for lyophilized products: They can be stored at 4°C for 24 months, and the liquid after reconstitution can be stored at -20°C for 6-12 months, avoiding repeated freeze-thaw cycles.

Expression Host: Human HEK293 cells
Source: Human
Synonyms: CD40LG, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39
Protein Sequence: The DNA sequence encodes human CD40 Ligand (UniProtKB/Swiss-Prot: P29965.1), expressed with a His tag at the C-terminus.
Molecular Weight: Recombinant human CD40 Ligand protein contains 149 amino acids, with a predicted theoretical molecular weight of 18.5 kD.
Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis.
Endotoxin: <0.1 EU/μg
Biological Activity: Immobilized human CD40 Ligand (His tag) at a concentration of 2 μg/mL (100 μL/well) can bind to human CD40 (FC tag) in a linear range of 0.2-2.5 μg/mL.
Purification Method: Chromatographic purification
Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.

Optimal Stability
Gmp-Grade Quality
Low Batch-to-Batch Variability
Exceptional Specificity
Robust Bioactivity
Efficient and Reliable Performance

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