Recombinant Human CEACAM-8/CD66b (His Tag), Yisheng (CAT#: STEM-CT-0193-ZJF)

The Recombinant Human CEACAM-8/CD66b (His Tag) manufactured by Yisheng is suitable for studying CEACAM-8-mediated cellular processes.
Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM-8), also known as CGM6 and CD66b, is an approximately 95 kDa member of the human carcinoembryonic antigen (CEA) family. CEACAM-8 plays a role in cell adhesion, migration, and pathogen binding. It is anchored to cells by a glycophosphatidylinositol (GPI) motif and is expressed exclusively on granulocytes. Activation enhances surface expression of CEACAM-8, making it an activation marker in human neutrophils and eosinophils. CEACAM-8 can form homotypic and heterotypic bonds with CEACAM-6, influencing cellular functions such as adhesion, superoxide production, and degranulation of eosinophils.

Synonyms: CD66b; CEACAM8; CGM6; CD67; NCA-95
Uniprot No.: P31997.2
Source: Recombinant Human CEACAM-8/CD66b Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Gln35-Ser319.
Molecular Weight: Approximately 32.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: > 95% as determined by SDS-PAGE and HPLC.
Activity: SPR Data: Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Human CEACAM-6, His Tag with an affinity constant of 4.59 μM as determined in SPR assay (Biacore T200).
Endotoxin: < 1.0 EU per 1μg of the protein by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Shipped with ice pack.
Stored at -20℃ to -80℃ for 1 year.
2-7 days, 2 to 8 ℃ under sterile conditions after reconstitution.
3 months, -20 ℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
For safety and health, please wear lab coats and disposable gloves for operation.

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