Recombinant Human DNAM-1 Protein, Seafrom (CAT#: STEM-CT-0113-ZJF)

The Recombinant Human DNAM-1 Protein manufactured by Seafrom is suitable for immune cell activation and cytotoxicity.
DNAX accessory molecule 1 (DNAM-1), also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), is a single-pass type I membrane protein that contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes, and a subset of T cells.
DNAM-1 plays a crucial role in mediating cellular adhesion to other cells that express its ligands, CD112 and CD155. Cross-linking DNAM-1 with antibodies results in cellular activation. Additionally, DNAM-1 can interact with PVR and PVRL2.
Storage Information:
Lyophilized samples can be stored at 4°C for 24 months. Once reconstituted, the liquid can be stored at -20°C for 6-12 months, with care taken to avoid repeated freeze-thaw cycles.

Expression Host: Human HEK293 cells
Source: Human
Synonyms: DNAM1, CD226, PTA1
Protein Sequence: The DNA sequence encodes human DNAM-1 (NCBI Reference Sequence: NP_006557.2), expressed with a His tag at the C-terminus.
Molecular Weight: Recombinant human DNAM-1 protein contains 229 amino acids, with a predicted theoretical molecular weight of 27.7 kD.
Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis.
Endotoxin: <0.1 EU/μg
Biological Activity: The ability to bind to CD155 (Fc tag) is confirmed by fixing human DNAM-1 (His tag) at 5 μg/mL (100 μL/well), with a linear range of 5-80 ng/mL.
Purification Method: Chromatographic purification
Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.

Optimal Stability
Gmp-Grade Quality
Low Batch-to-Batch Variability
Exceptional Specificity
Robust Bioactivity
Efficient and Reliable Performance

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