Recombinant Human IL-3 Protein, Seafrom (CAT#: STEM-CT-0014-ZJF)

The Recombinant Human IL-3 Protein manufactured by Seafrom is suitable for supporting the growth and differentiation of hematopoietic cells.
IL-3 is a hematopoietic growth factor that promotes the survival, differentiation, and proliferation of progenitor cells in megakaryocytes, granulocytes-macrophages, red blood cells, eosinophils, basophils, and mast cells. It is produced by T cells, mast cells, and eosinophils and enhances platelet production, phagocytosis, and antibody-mediated cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory effects. Many of the activities of IL-3 depend on synergistic stimulation with other cytokines. IL-3 is a species-specific and variably glycosylated cytokine.
Lyophilized preparations can be stored at 2~8°C for 24 months. For aliquoting, they can be reconstituted with injection water, physiological saline, culture medium, or PBS. After reconstitution, divide into smaller portions and store at -20°C for 6 months or at -80°C for 12 months. The final concentration after dilution should not be lower than 100μg/mL. Avoid repeated freeze-thaw cycles.

Expression Host: HEK293 Cells
Synonyms: MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor
Protein Sequence: DNA sequence encodes human IL-3 (GenBank: AAA59146.1) expressed with a C-terminal His tag
Molecular Weight: Recombinant human IL-3 protein consists of 139 amino acids with a predicted molecular weight of 15.9 kD
Purity: >90%, analyzed by SDS-PAGE
Endotoxin Level: <0.1 EU/μg
Biological Activity: Cell proliferation assay using TF-1 cells, activity ≥1.0×10^6 U/mg
Purification Method: Chromatographic purification
Appearance: White loose body

Stable Cell Library
Large-Scale Expression
High Activity
Batch Stability
Gmp-Quality Control
Ensured Product Quality

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