Recombinant Human Sigle2/CD22 (His Tag), Yisheng (CAT#: STEM-CT-0203-ZJF)

The Recombinant Human Sigle2/CD22 (His Tag) manufactured by Yisheng is suitable for studying Siglec-2/CD22-mediated immune responses.
Siglec-2, also known as B-cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B-cell restricted glycoprotein expressed in progenitor B and pre-B cells and on the surface of mature B cells. The predominant Siglec-2/CD22 beta encodes an 847 amino acid polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, six Ig-like C2-type domains, a transmembrane region, and a cytoplasmic tail with 4 immunoreceptor tyrosine-based inhibition motifs (ITIMs). Siglec-2/CD22 is a coreceptor that physically interacts with B-cell receptor (BCR) and negatively regulates BCR signals by recruiting tyrosine phosphatase SHP-1 to its ITIMs. Phosphorylated Siglec-2/CD22 can interact with other intracellular effector proteins, such as Syk, PLC gamma, PI3 kinase, and Grb-2, suggesting it may play a role in positive signaling.

Synonyms: CD22 molecule, Siglec-2, B-cell receptor CD22, BL-CAM, SIGLEC2, SIGLEC2FLJ22814
Uniprot No.: P20273-1
Source: HEK293 Cells-derived Human Siglec-2 / CD22 Protein, Asp20-Arg687 with His tag at the C-terminal
Molecular Weight: Approximately 76.2 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: > 95% as determined by SDS-PAGE and HPLC
Activity: ELISA Data: Immobilized Human Siglec-2, His Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 19.1 ng/mL determined by ELISA.
Endotoxin: < 1.0 EU per 1μg of the protein by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Shipped with ice pack.
Stored at -20℃ to -80℃ for 1 year.
2-7 days, 2 to 8 ℃ under sterile conditions after reconstitution.
3 months, -20 ℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
For safety and health, please wear lab coats and disposable gloves for operation.

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