Recombinant Human TIGIT Protein, Seafrom (CAT#: STEM-CT-0151-ZJF)

The Recombinant Human TIGIT Protein manufactured by Seafrom is suitable for immune checkpoint regulation.
T-cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9) and V-set and transmembrane domain-containing protein 3 (VSTM3), is a single-pass type I membrane protein. It contains an immunoglobulin variable domain, a transmembrane domain, and an immunoreceptor tyrosine-based inhibitory motif (ITIM).
TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and natural killer (NK) cells. Its expression is up-regulated following activation of these cells. TIGIT binds with high affinity to the poliovirus receptor (PVR), leading to increased secretion of interleukin-10 (IL-10) and decreased secretion of interleukin-12B (IL-12B). It suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
The freeze-dried (lyophilized) form of TIGIT can be stored at 4℃ for up to 24 months. Once reconstituted, the liquid can be stored at -20℃ for 6-12 months, with precautions taken to avoid repeated freeze-thaw cycles.

Expression Host: Human HEK293 cells
Source: Human
Synonyms: VSIG9, VSTM3
Protein Sequence: The DNA sequence encodes human TIGIT (NCBI Reference Sequence: NP_776160.2), expressed with an Fc tag at the C-terminus.
Molecular Weight: Recombinant human TIGIT protein contains 351 amino acids, with a predicted theoretical molecular weight of 40 kD.
Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis.
Endotoxin: <0.1 EU/μg
Biological Activity: Immobilized human TIGIT (His tag) at 1 μg/mL (100 μL/well) can bind to human CD155 (His tag) in a linear range of 10-100 ng/mL.
Purification Method: Chromatographic purification
Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.

Optimal Stability
Gmp-Grade Quality
Low Batch-to-Batch Variability
Exceptional Specificity
Robust Bioactivity
Efficient and Reliable Performance

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