Reverse Yeast Two-hybrid (rY2H) Library Construction to study protein-protein interactions (CAT#: STEM-MB-0021-WXH)

Introduction

Protein-protein interactions take part in most biological processes of cells including gene expression, cell growth, proliferation, morphology, motility, intercellular communication. And the use of yeast two-hybrid (Y2H) system has provided definition to previously unknown pathways by the characterization of novel interactions between proteins. The two-hybrid system relies on the bifunctional nature of transcription factors, such as yeast enhancer Gal4 or repressor LexA to allow protein-protein interactions to be monitored through transcriptional changes of reporter genes. When a positive interaction has been validated, either of the interacting proteins can be mutated by site-specific or randomly introduced changes, to generate proteins with a decreased capability to interact. Such technique is termed reverse two-hybrid (rY2H/rYTH) method, specifically designed to promote identification of events isolating protein-protein interactions. More attention will shift to this powerful search engine for the establishment of interaction networks as well as drug discovery in understanding of diseases.