Ribosome-profiling (Ribo-Seq) (CAT#: STEM-MB-0077-WXH)

Introduction

Ribosome-profiling techniques were developed in response to the importance of measuring gene expression at the translational level. Ribosome profiling is a direct technique for determining the precise mRNA region read by the ribosome and for directly examining the transcripts associated with the translation machinery. In comparison to conventional RNA-Seq studies, ribosome profiling is created to assess mRNA included in inactive translation.
The reality that translating ribosomes can protect about 30 nucleotides of an mRNA fragment from RNAse digestion allows for ribosome profiling. The nuclease is used to destroy unprotected regions of in vitro translated mRNAs. The ribosome-protected mRNAs can be plotted back to the initial mRNA to assess the precise location of the translating ribosome.