RLRs Signaling Pathway Assay (CAT#: STEM-MB-0356-WXH)

Introduction

RIG-I-like receptors (retinoic acid-induced gene I-like receptors, RLR) are a family of molecules that are expressed in cells to sense viruses and belong to pattern recognition receptors for innate immunity. As a type of RNA helicase in the cytoplasm, they can recognize non-self viral RNA by combining their RNA ligands with pathogen-related molecular patterns. In infected cells, this interaction can trigger the fire activation of RLRs and downstream signaling molecules, which ultimately leads to the production of type I interferon and the production of inflammatory factors, and the cells make an antiviral immune response.

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Principle Applications Procedure Materials Notes Related Products

Principle

In the antiviral signal pathway of RIG-I-like receptors, most of the reason is that RIG-I-like receptors can recognize viral components and induce the production of type 1 interferon, relying on its protein structure to transmit viral signals. The RIG-I-like receptor has three domains: the repeat domain (responsible for recognizing PAMPs), the transmembrane domain, and the TIR receptor domain that transmits activation signals. The TIR receptor domain can recruit TIR-containing domains. Adaptor proteins, such as TRIF and MyD88 are common adaptor proteins. RIG-I plays an important role in animal virus infection.

Applications

To study the effects of drugs or therapies on RIG-I-like receptor signaling pathways
To study the effect of each virus on RIG-I-like receptor signaling pathway
To study the regulation mechanism of RIG-I-like receptor signaling pathway in disease

Procedure

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Notes

Detectable targets: c-Cb1, CYLD, RNF125, K48, MDA5, NLRX1, MAVS, Gp78, K63, TRAF2, TRAF6, TRAF3, FADD, TBK1, RIPI, NEMO, IKK-α, IKK-β, IkBα, NFκB, IRF3, IRF7, IKKi, IFN-α, IFN-β

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