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S-PLEX is an ultrasensitive assay platform. It can dramatically improve the sensitivity of immunoassays, thereby reducing the lower limit of detection (LLOD) by 10- to 1000-fold over other assay methods. Detection limits in the low femtogram/mL range are common. These low detection limits enable the measurement of analytes at lower concentrations, reduce required sample volume, and reduce critical reagent usage.
The increased sensitivity of S-PLEX assays has important implications. S-PLEX shifts the dynamic range of assays, resulting in low detection limits. S-PLEX assays can measure new analytes that were previously below the detectable range of any existing assays, enabling the discovery and use of new biomarkers. S-PLEX assays have been tested with serum, plasma and cell culture supernatants.