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S-PLEX Services as an ultrasensitive assay platform (CAT#: STEM-MB-0244-WXH)

Introduction

S-PLEX is an ultrasensitive assay platform. It can dramatically improve the sensitivity of immunoassays, thereby reducing the lower limit of detection (LLOD) by 10- to 1000-fold over other assay methods. Detection limits in the low femtogram/mL range are common. These low detection limits enable the measurement of analytes at lower concentrations, reduce required sample volume, and reduce critical reagent usage.
The increased sensitivity of S-PLEX assays has important implications. S-PLEX shifts the dynamic range of assays, resulting in low detection limits. S-PLEX assays can measure new analytes that were previously below the detectable range of any existing assays, enabling the discovery and use of new biomarkers. S-PLEX assays have been tested with serum, plasma and cell culture supernatants.




Applications

Study biomarkers that were previously unmeasurable.

Procedure

The S-PLEX procedure is comprised of three simple steps.
1. ASSEMBLE — is to assemble the immunoassay in an plate.
2.ENHANCE — involves adding S-PLEX reagents that aid in the enhancement and generation of signal.
3.READ — is simply the addition of Read Buffer and measurement of the assay signals on an instrument.
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