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Seahorse XFe24 Analyzer (CAT#: AM-LE-0325-Y)

Highlights

• The integrated system can report metabolic rate in minutes without sample extraction or labeling
• Wave software can control the instrument and perform real-time rate measurement to provide results on the day of measurement
• Real-time detection of the response of substrates, inhibitors and other compounds, dosing and automatic mixing through the 4 dosing port system while maintaining physiological temperature (37°C)

Cat Number: AM-LE-0325-Y

Application: • Can analyze islet function or other flowing samples<br />• Test to detect mitochondrial function<br />• Simultaneous quantitative analysis of ATP produced by glycolysis and oxidative phosphorylation<br />• A metabolic phenotype can be generated within an hour<br />• Measure the rate of glycolysis in living cells<br />• Quickly detect the dependence of cellular energy generation on mitochondrial substrates

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Description

Seahorse XFe24 Analyzer is manufactured by Agilent Technologies, used to determine the OCR and ECAR of live cells in 24-well plates, so as to understand the cell metabolism function of cultured cells and in vitro samples.

Specification

Number of analysis holes: 20–22
Microchamber volume: 7 µL
Plate specifications: 24-well microplate
Controller: Windows 10 computer combined with a touch screen display with complete test design, control and analysis functions.
Software: Design and run analysis templates on Wave Controller or Wave Desktop.
Main advantages:
Verify under hypoxic conditions
Verification at a detection temperature of 16–42 °C (requires a temperature below room temperature of 12–20 °C)

Features

• The integrated system can report metabolic rate in minutes without sample extraction or labeling
• Wave software can control the instrument and perform real-time rate measurement to provide results on the day of measurement
• Real-time detection of the response of substrates, inhibitors and other compounds, dosing and automatic mixing through the 4 dosing port system while maintaining physiological temperature (37°C)
• Analyze only 10,000 cells per well in a custom 24-well plate The cell number requirements vary according to cell type
• Has a precision temperature-controlled heating tray that can maintain 16-42 °C (12 °C-20 °C above room temperature), so it is compatible with a variety of samples

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