Sephacryl S-300 HR, 750 mL, Cytiva (CAT#: STEM-C-1623-LC)

Sephacryl S-300 High Resolution manufactured by Cytiva is a highly versatile gel filtration resin which is ideal for fast and reproducible purification of antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 1 × 10⁴-1.5 × 10⁶) by size exclusion chromatography. The matrix of Sephacryl High Resolution resins is a cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide. This crosslinking gives good rigidity and chemical stability. The combination of narrow particle size distribution and steep selectivity curves offers good preparative characteristics and high resolution. The hydrophilic nature of the resin reduces nonspecific adsorption and increases recovery.

BioProcess resin: Yes
Particle size, d50V1: ~50 µm
Matrix: Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.
pH stability, operational2: 3-11
pH stability, CIP3: 2-13
Pressure/flow characteristics4: Pressure/flow characteristics: ≥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20°C using buffers with a viscosity similar to water).
Fractionation range (Mr) (globular proteins): 1 × 10⁴-1.5 × 10⁶
Fractionation (Mp) Dextrans: 2 x 10³-4 x 10⁵
Fractionation Range (Mr) DNA Exclusion Limit: 118 bp
Exclusion limit (Mr) (globular proteins): ~2 × 10⁶ (2000 kDa)
Storage: 4 to 30°C, 20% Ethanol
Chemical stability: Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl
Certificate of Analysis: Yes
Pack size: 750 mL

High resolution
Fast and easy operation
Long-term physical and chemical stability

Sephadex G-25 Fine, 100 g, Cytiva (CAT#: STEM-C-1592-LC)