Sephadex G-15 manufactured by Cytiva is a well established gel filtration resin for desalting and buffer exchange of peptides and small biomolecules>1500 molecular weight. Sephadex G-15 is prepared by crosslinking dextran with epichlorohydrin. Sephadex G-15 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules.
Specification
Matrix: Cross-linked dextran Particle size distribution, wet: 60-180 µm Particle Size Dry: 40 µm-120 µm pH stability, operational1: 2-13 Flow rate2: < Darcy´s Law Pressure/Flow Specification: 20-47 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d. Fractionation range (Mr) (globular proteins): <1.5 × 10³ Fractionation (Mp) Dextrans:>< 1.5 x 10³ Storage: 4 to 30°C Autoclavable: In wet form (pH 7.0) at 120°C for 30 min Pack size: 100 g