Sephadex G-50 Medium, 500 g, Cytiva (CAT#: STEM-C-1608-LC)

Sephadex G-50 manufactured by Cytiva is a well established gel filtration resin for purification of DNA from small molecules. Sephadex G-50 is prepared by crosslinking dextran with epichlorohydrin. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances.

Matrix: Cross-linked dextran
Particle size distribution, wet: 100-300 µm
Particle Size Dry: 50 µm-150 µm
Application: For the separation of molecules Mr>30 000 from molecules Mr <1500 such as labeled protein or DNA from unconjugated dyes For removeing small nucleotides from longer chain nucleic acids
pH stability, operational1: 2-10
pH stability, CIP2: 2-13
Flow rate: < Darcy´s Law
Pressure/Flow Specification: min 150 cm/h, bed height 10 cm, column 5 cm i.d.
Fractionation range (Mr) (globular proteins): 1 × 10³-3 × 10⁴
Fractionation (Mp) Dextrans: 5 x 10²-1 x 10⁴
Exclusion Limit:> 3 x 10⁴
Storage: 4 to 30°C
Autoclavable: In wet form (pH 7.0) at 120°C for 30 min
Pack size: 500 g

Sephacryl S-100 HR, 750 mL, Cytiva (CAT#: STEM-C-1631-LC)