Sephadex G-50 manufactured by Cytiva is a well established gel filtration resin for purification of DNA from small molecules. Sephadex G-50 is prepared by crosslinking dextran with epichlorohydrin. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances.
Specification
Matrix: Cross-linked dextran Particle size distribution, wet: 20-80 µm Particle Size Dry: 20 µm-50 µm pH stability, operational1: 2-10 Flow rate2: < Darcy´s Law Pressure/Flow Specification: min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d. Fractionation range (Mr) (globular proteins): 1 × 10³-3 × 10⁴ Fractionation (Mp) Dextrans: 1 x 10²-5 x10³ Exclusion Limit:> 3 x 10⁴ Storage: 4 to 30°C Autoclavable: In wet form (pH 7.0) at 120°C for 30 min Pack size: 100 g