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Scopolamine is a natural tropane alkaloid widely used for medicinal purposes due to its anticholinergic and antispasmodic effects. However, this compound is also used as a potent sedative that facilitates sexual crimes when it is intentionally mixed into drinks. Butylscopolamine – active ingredient of buscopan® – is a safer compound derived from scopolamine and commonly used for treatments of abdominal pain and intestinal spasms. This service provides a method based on capillary electrophoresis with capacitively coupled contactless conductivity detection for rapid (∼17 injections h−1) and simultaneous quantification of scopolamine and butylscopolamine using a buffer solution composed by 20 mmol L−1 butyric acid and 7 mmol L−1 NaOH (pH = 4.5).