Single-Cell Proteomics

Single-cell proteomics provides us with an in-depth knowledge about phenotypic heterogeneity of individual cells and cell specific functional networks underlying biological processes. Single-cell proteomics profiling has been majorly divided into targeted single-cell proteomics and global single-cell proteomics. Targeted single-cell proteomics refers to detection of the secreted and intracellular proteins by using labeled affinity probes such as antibodies. On the other hand, global single-cell proteomics uses mass spectrometry (MS) as the main tool for analysis. The main process of single-cell proteomics, comprising single-cell sorting/isolation, proteomic sample preparation, and proteomics detection.

To obtain high sensitivity and facilitate proteomics identification at the single-cell level, microfluidics has been increasingly applied, because it features several advantages, such as controllable cell manipulation, substantially reduced reagent consumption, and design flexibility to increase multiplexity and throughput. Microfluidics can be integrated to the following downstream detection techniques to complete single-cell proteomics analysis:

• Flow cytometry/imaging flow cytometry
• Microengraving
• Capillary electrophoresis
• Western blotting
• MS

What We Can Do for You

STEMart utilizes microfluidics-based toolkit to detect functional proteomics at a single cell level service to facilitate the following researches:

• Measure heterogeneity in different cell lines and dissect tissue heterogeneity
• Measure functional heterogeneity in cellular immunity
• Explore the subcellular distribution of proteins in individual cells
• Analysis of cell behavior
• Explore cellular interactions and communications
• Explore cancer development and progression
• Identify biomarkers
• Unveil cellular responses to drugs
• Investigate mechanistic insights into drug resistance
• Discover functional proteins to track cellular phenotypes
• Analyze cellular signaling events
• Characterize interactions between protein and protein

For more information about our single-cell proteomics service, please contact us.