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In this service, simultaneous separation and detection of three organomercury species, namely methylmercury (MeHg), ethylmercury (EtHg), and phenylmercury (PhHg), is performed by using capillary electrophoresis (CE) with UV detection. Pre-column derivatization with thiosalicylic acid and on-line salt-induced stacking significantly improve the detection performance. Buffer pH, ion strength, and additive are optimized for CE separation, concentration of NaCl in sample solution and injection time are optimized for on-line stacking.