Spectral karyotype analysis of T-cell acute leukemia (CAT#: STEM-ST-0377-WXH)

Introduction

T-cell acute lymphoblastic leukaemia (T-cell ALL) is an acute leukaemia in which too many abnormal T-cells are produced in the bone marrow. T-cell ALL is a more aggressive subtype of ALL compared with B-cell ALL.

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Principle Applications Procedure Materials

Principle

It is based on the hybridization of collections of chromosome-specific FISH probes labeled with different fluorochromes or fluorochrome combinations, allowing the discrimination of each of the 24 different human chromosomes.

Applications

Detection of subtle chromosomal aberrations, including marker chromosomes, small translocations, complex rearrangements and minute structural abnormalities.

Procedure

1. Metaphase preparation
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis

Materials

Fluorescent dyes, spectrum orange, Texas red, Cy5, spectrum green, Cy5

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