Spectral karyotyping study of chromosome abnormalities in human leukemia (CAT#: STEM-ST-0375-WXH)

Introduction

Acute myelogenous leukemia (AML) with high numbers of blasts is often associated with complex chromosomal abnormalities and multiple markers. Specific identification of these chromosomal abnormalities plays an important role in diagnosis, prognosis, and selection of treatment for patients.

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Principle Applications Procedure Materials Notes Related Products

Principle

It is based on the hybridization of collections of chromosome-specific FISH probes labeled with different fluorochromes or fluorochrome combinations, allowing the discrimination of each of the 24 different human chromosomes.

Applications

Detection of subtle chromosomal aberrations, including marker chromosomes, small translocations, complex rearrangements and minute structural abnormalities.

Procedure

1. Metaphase preparation
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis

Materials

Fluorescent dyes, spectrum orange, Texas red, Cy5, spectrum green, Cy5

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