Stain mouse brain tissue by Nissl staining technology (CAT#: STEM-CT-0006-LJX)

Introduction

There are a large number of basophilic structures in neurons, which are composed of parallel rough endoplasmic reticulum and poly ribosome, called Nissl body, also called tiger spot or leopard spot. Their shape, size and number are different, so the number and shape of Nissl body can also reflect the functional state of neurons.
Observing the state of the Nissl body in mouse brain tissue cells by staining can reflect the function of brain neurons.

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Principle Applications Procedure Materials Notes Related Products

Principle

Nissl Staining was a method of staining nerve tissues with basic dyes. Nissner staining can be used to stain Nissner bodies, which can be used to observe the cellular structure of neurons. It can also be used to understand the damage of neurons by observing Nissner bodies. The main basic dyes used for Nissl Staining are tar purple, methylene blue, toluidine blue and thionin.

Applications

Staining of nerve tissue section samples from experimental animals

Procedure

1.Paraffin section dewaxing
2.Clean the slices
3.Staining
4.Rinse with pure water
5.Wash with ethanol
6.Dip the slices into the color separation solution for color separation
7.Wash with ethanol
8.Xylene transparent
9.Seal the slices

Materials

• Sample Type:
Mouse brain tissue

Notes

1.The staining and color separation steps can be repeated until satisfactory results are achieved.
2.Dehydrate thoroughly before sealing.

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Stain neuronal cell bodies by Nissl staining technology (CAT#: STEM-CT-0005-LJX)