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There are a large number of basophilic structures in neurons, which are composed of parallel rough endoplasmic reticulum and poly ribosome, called Nissl body, also called tiger spot or leopard spot. Their shape, size and number are different, so the number and shape of Nissl body can also reflect the functional state of neurons.
In 1892, German pathologist Nissl F (1860-1919) stained neurons with alkaline dyes, thus discovering Nissl bodies and establishing the Nissl staining method. In Nissl staining, Nissl bodies are clearly distinguishable, and the nucleus and fine nucleoli are also very clear. It is also easy to distinguish axons and dendrites, achieving the effect of both identifying organs and observing special cytoplasmic structures.