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Stain neuronal cell bodies by Nissl staining technology (CAT#: STEM-CT-0005-LJX)

Introduction

There are a large number of basophilic structures in neurons, which are composed of parallel rough endoplasmic reticulum and poly ribosome, called Nissl body, also called tiger spot or leopard spot. Their shape, size and number are different, so the number and shape of Nissl body can also reflect the functional state of neurons.
In 1892, German pathologist Nissl F (1860-1919) stained neurons with alkaline dyes, thus discovering Nissl bodies and establishing the Nissl staining method. In Nissl staining, Nissl bodies are clearly distinguishable, and the nucleus and fine nucleoli are also very clear. It is also easy to distinguish axons and dendrites, achieving the effect of both identifying organs and observing special cytoplasmic structures.




Principle

Nissl bodies can combine with alkaline dyes to produce color, and its quantity and plaque size can reflect the functional status of neurons.In Nissl staining, the Nissl body appears as a block (shaped like a tiger spot) or granule after being stained. The Nissl body particles around the nucleus are larger, and they are smaller and slender near the edge. Under physiological conditions, the large and numerous Nissl bodies indicate that neurons have a strong ability to synthesize proteins; When neurons are damaged, the number of Nissl bodies can decrease or even disappear.

Applications

Staining of nerve tissue section samples from experimental animals

Procedure

1.Paraffin section dewaxing
2.Clean the slices
3.Staining
4.Rinse with pure water
5.Wash with ethanol
6.Dip the slices into the color separation solution for color separation
7.Wash with ethanol
8.Xylene transparent
9.Seal the slices

Materials

• Sample Type:
Nerve tissue section samples from experimental animals

Notes

1.The staining and color separation steps can be repeated until satisfactory results are achieved.
2.Dehydrate thoroughly before sealing.

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