Study of Dynamic Coupling Between DNA and its Primary Hydration Shell by Brillouin Scattering (CAT#: STEM-ST-0141-YJL)

Introduction

The structure and dynamics of water surrounding DNA, proteins, and membranes have been the subject of intense experimental and theoretical study. It has been argued that the most important vibrations of a biopolymer lie in the GHz to a few hundred GHz frequency range. Because the relaxation frequencies of the hydration shells lie in this frequency range, water cannot be treated as a viscous damping medium (low-frequency case) or some perturbation on the local bonds (high-frequency case). Some dieletric, and inelastic neutron-scattering measurements have been used to study the hydration shells of DNA in this frequency range.

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Principle Applications Procedure Materials Notes Related Products

Principle

From a quantum point of view, Brillouin scattering is an interaction of light photons with acoustic or vibrational quanta (phonons), with magnetic spin waves (magnons), or with other low frequency quasiparticles interacting with light. The interaction consists of an inelastic scattering process in which a phonon or magnon is either created (Stokes process) or annihilated (anti-Stokes process). The energy of the scattered light is slightly changed, that is decreased for a Stokes process and increased for an anti-Stokes process. This shift, known as the Brillouin shift, is equal to the energy of the interacting phonon and magnon and thus Brillouin scattering can be used to measure phonon and magnon energies.

Applications

Brillouin scattering is used to determine acoustic velocities and elastic properties of a number of crystalline solids, glasses, and liquids.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Brillouin scattering measurement system (Brillouin spectrometer)

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