Study of Interaction between a cationic porphyrin and ctDNA by UV-Vis Spectroscopy (CAT#: STEM-MB-0896-WXH)

Introduction

Cationic porphyrin is considered as a dual functional drug that can strongly bind to DNA as well as photodynamically modify the target site of DNA molecule by a mechanism similar to that of anticancer antibiotics, such as blemycin and daunomycin. The interaction between porphyrins and DNA involves key-binding modes such as intercalation, outside self-stacking, and outside random binding.

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Principle Applications Procedure Materials Notes Related Products

Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer

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