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Study of molecular orientation in model membrane systems by Linear dichroism (LD) (CAT#: STEM-ST-0129-WXH)

Introduction

The most basic function of biological membranes is to define a boundary, whether between or within cells and organelles. Many cellular processes depend upon the membrane’s ability to separate different areas while allowing communication and tightly regulated transport within and across membranes to occur.
Because they play such a fundamental role and because natural membranes are so complex, many different model systems have been created that retain the essential lipid bilayer structure, but simplify the system so that the roles of individual components can be assessed and that their organization and dynamics can be visualized.




Principle

Linear dichroism (LD) or diattenuation is the difference between absorption of light polarized parallel and polarized perpendicular to an orientation axis. It is the property of a material whose transmittance depends on the orientation of linearly polarized light incident upon it. LD measurements are based on the interaction between matter and light and thus are a form of electromagnetic spectroscopy.

Applications

Study the functionality and structure of molecules.

Materials

• Polarizer
• Absorbance spectrometer
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